Twice Europa League winners Porto will face two-times finalists AS Roma in a knockout phase playoff tie, while Jose Mourinho's Fenerbahce were pitted against Anderlecht in Friday's draw held in Nyon, Switzerland.
Porto, winners in 2003 and 2011, last met Serie A club Roma in a Champions League last-16 clash in 2019, in which the Portuguese side emerged victorious.
Turkey's Fenerbahce face Belgium's Anderlecht, while fellow Super Lig club Galatasaray take on AZ Alkmaar. Ajax Amsterdam, champions in 1992, will meet Union SG and FC Twente play Bodo/Glimt of Norway.
The top eight sides automatically qualified for the last 16 while the teams that finished ninth to 24th will compete in the two-legged playoffs.
Premier League sides Manchester United and Tottenham Hotspur, among the favourites to win the tournament, secured automatic qualification to the Europa League last 16.
United and Spurs could face the winners of the tie between Midtjylland and Real Sociedad, or the winners of AZ Alkmaar v Galatasaray.
EUROPA LEAGUE KNOCKOUT PLAYOFFS DRAW
- FC Twente v Bodo/Glimt
- Union SG v Ajax Amsterdam
- AZ Alkmaar v Galatasaray
- Ferencvaros v Viktoria Plzen
- Fenerbahce v Anderlecht
- PAOK v Steaua Bucharest
- Midtjylland v Real Sociedad
- Porto v AS Roma
The playoff first leg will be played on February 13 and the second leg on February 20. The draw for the last-16 stage will be held on February 21.
Dubai to spotlight future of sport at World Sports Summit
Safeguarding rules bar 15-year-old Sooryavanshi from Indian dressing room
Top contender Sabalenka faces fresh scrutiny at Wimbledon as mental scars linger
Munoz strike sends Colombia into World Cup Round of 32
Qatar's sports minister, Madibo visit Canada's injured Kone
